5 (E11.5) in mouse. Using a machine-learning approach to integrate the data from different contexts, I found that E11.5 heart enhancers can often be predicted accurately from data from other contexts, and I quantified the contribution AZD9291 nmr of each data source to the predictions. The utility of each dataset correlated with nearness in developmental time and tissue to the target context: data from late

developmental stages and adult heart tissues were most informative for predicting E11.5 enhancers, while marks from stem cells and early developmental stages were less informative. Predictions based on data collected in non-heart tissues and in human hearts were better than random, but worse than using data from mouse hearts. Conclusions: The ability of these algorithms to accurately

predict developmental enhancers based on data from related, but distinct, cellular contexts suggests that combining computational models selleck kinase inhibitor with epigenetic data sampled from relevant contexts may be sufficient to enable functional characterization of many cellular contexts of interest.”
“The effect on hydraulic conductivity in porous media of CaCO3 precipitation induced by Sporosarcina pasteurii (ATCC 11859) was investigated using continuous-flow columns containing glass beads between 0.01 mm and 3 mm in diameter. Resting S. pasteurii cells and a precipitation solution composed of 0.5 M CaCl2 and 0.5 M urea were

introduced into the columns, and it was shown that the subsequent formation of CaCO3 precipitation reduced hydraulic conductivity from between 8.38 x 10(-1) and 3.27 x 10(-4) cm/s to between 3.70 x 10(-1) and 3.07 x 10(-5) cm/s. The bacterial KU-57788 in vitro cells themselves did not decrease the hydraulic conductivity. The amount of precipitation was proportional with the bacterial number in the column. The specific CaCO3 precipitation rate of the resting cells was estimated as 4.0 +/- 0.1 x 10(-3) mu g CaCO3/cell. Larger amounts of CaCO3 precipitation were deposited in columns packed with small glass beads than in those packed with large glass beads, resulting in a greater reduction in the hydraulic conductivity of the columns containing small glass beads. Analysis using the Kozeny-Carman equation suggested that the effect of microbially induced CaCO3 precipitation on hydraulic conductivity was not due to the formation of individual CaCO3 crystals but instead that the precipitate aggregated with the glass beads, thus increasing their diameter and consequently decreasing the pore size in the column. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.”
“We recently developed a clinical grade ex vivo cord blood expansion procedure enabling a massive amplification of hematopoietic progenitors without any loss of stem cell potential.