00, min 23, and max 29, where �� is the relative selleck chemicals Enzastaurin intensity threshold for significant expression, min is the minimum number of significant expression in the experiment set, and max is the maximum number of significant expression in the control set. There are 69 gene targets identified for potential liver selective expression, and the priority score ranges from 1. 64 to 5. 88. Based on the permutation analysis, the liver selective expression patterns of all the selected genes are statisti cally significant. The expression patterns of these genes are shown in Figure 3. Interestingly, 17 of the top 20 high scoring genes listed in Table 3 are previously known to be expressed predominantly in the liver. In particular, nine genes are highly expressed in the liver, and their protein products are secreted to blood plasma.

MASP2, CFHR5, CFHR3, CRP, CFHR4 and MBL2 play important roles in the innate immune defense against pathogens. SERPINC1 and F2 are involved in regu lating the blood coagulation cascade. APOA5 encodes an apolipoprotein important for the regulation of plasma triglyceride level, a major risk factor for cor onary artery disease. Six of the known liver selec tive genes encode metabolic enzymes involved in cholesterol catabolism and bile acid biosynthesis, the urea cycle, glyoxylate detoxifica tion, and the oxidation of alcohols and other compounds. In addition, HGFAC encodes a peptidase involved in hepatocyte growth factor activation, and C14orf68 encodes a liver specific mitochondrial carrier protein. The other three high scoring genes have not been previously shown to be expressed preferentially in the liver.

Testis selective gene expression When compared with brain and liver tissues, many other tissues have fewer number of microarray expres sion profiles available. The microarray dataset has only 36 expression profiles of the testis, which pro duces sperm and male sex hormones. To identify testis selective genes, these 36 expression profiles were compared with 2,932 microarray profiles of non testis tissues by using the following parameters, �� 1. 00, min 7, and max 29. The analysis resulted in 581 gene targets with the priority score ranging from 1. 35 to 6. 05. The testis selective expression patterns of these targets were found to be statistically significant by permutation testing. Figure 3 shows the expression patterns of the testis selective gene targets.

As listed in Table 4, the top 20 high scoring targets include five known testis selective genes. The C9orf11 gene encodes a vesicle membrane protein involved in the biogenesis of acrosome, a cap like structure that covers the anterior half of the head in the spermatozoa. TNP2 encodes a chromosomal transition protein for the conversion of nucleosomal chromatin to the compact form found Drug_discovery in the sperm nucleus.