1 mg/ml) remained stable when stored at refrigerator conditions for 7 days including the storage at room temperature for 8 h. Donepezil was stable in plasma samples when stored at room temperature for 19 h. Donepezil was found to be stable for three freeze and thaw cycles. Donepezil was stable and did not show any degradation when stored in the freezer for 96 days. Donepezil in the processed samples was stable for 82 h when stored in the auto sampler at 10 °C. The method characteristics are represented in Table 2. We described here the development of a new, selective, precise and accurate method for the quantification of

donepezil in human plasma using Liquid Chromatography Mass Spectrometric method with the simple liquid–liquid extraction technique using the less volume of plasma and is suitable for application to a pharmacokinetic, bioequivalence and drug interaction studies for the estimation of donepezil from plasma. this website The limit of quantification of the method was set to 50 pg/ml considering the dosage of donepezil

administered and selleck chemicals it is determined not only by detection technique but also by the effective clean-up of sample and thus improving the signal to noise ratio. The method reported here uses a simple and effective extraction technique with good and reproducible recovery. All authors have none to declare. Authors are thankful to JPR Solutions for providing the support during publication. “
“Wound healing is a complex issue involving many sequential steps in the restoration of the skin to its normal state.1 Wound healing process is delayed by the free radicals present in the wound. The free radicals act by causing lipid peroxidation, enzymatic degradation & DNA breakage. Moreover the exposure of the wound to the external environment worsens the phases of wound healing since it is prone to the microbial attack. There is a substantial evidence of the role of antioxidants acting against the free radicals by scavenging Adenylyl cyclase them.2 Natural extracts like Centella asiatica, Terminalia arjuna are renowned for their

antioxidant properties. 3 They possess the active constituents such as quinones, iridoids, triterpenoid derivatives, coumarins, flavonoids and isoflavonoids which play an important role in wound healing. 4, 5 and 6 The applicability of these extracts as such, is a point to be considered as inappropriate concentration may lead to subtherapeutic or undesired effects. At this juncture the need of a suitable delivery system for this extracts is inevitable. In this research an attempt was made to impregnate these extracts in a collagen matrix (a natural polymer) which acts as a base or platform by providing the physical support and ensuring the slow release of the extract. 12 and 14 Further, collagen is interlinked with the wound healing property by the virtue of its nature. Other advantages of the usage of collagen include its biodegradability & biocompatibility.