1b). Using multiple regression analysis, we evaluated independent effects of genetic and non-genetic factors on the development of thyroid autoantibodies. The reference categories for the analysis were CT60 CTLA-4 genotype, age, family history of AITD and cigarette smoking. In the case of thyroid peroxidase antibodies, selleck chemicals we confirmed a significant contribution of CT60 CTLA-4 genotype (P < 0·007) and younger age (P < 0·05), while family history and cigarette smoking did not prove to have any effect. In thyroglobulin antibodies, no contribution of either genotype or non-genetic factors
was confirmed. The genotyping in the group of 75 PPT patients revealed the AA genotype in 17 (22·7%) patients, the AG genotype in 36 (48%) and the GG genotype in 22 (29·3%) patients, showing no deviation from HWE (χ2 0·096, P = 0·757). As presented in Table 2, the patients with different genotypes did not differ in age, number of pregnancies, family history of AITD and smoking status. However, females with the G-allele carrying genotypes presented significantly more often with positive values of thyroid peroxidase antibodies (P < 0·04), while
the proportion of thyroglobulin antibody-positive patients did not differ significantly between the three genotypes. Similarly, more patients with the G-allele carrying genotypes had at least one type of thyroid autoantibody elevated compared
to the AA genotype (P < 0·04) (Table 2). Furthermore, the median value of thyroid peroxidase antibodies was Y-27632 chemical structure significantly lower in the AA genotype compared to the AG and GG genotypes (median, 12, 130 and 423 U/ml, respectively, P < 0·006) (Fig. 2a). In contrast to thyroid peroxidase antibodies, the median values of thyroglobulin antibodies did not differ significantly between the three genotypes (Fig. 2b). For the evaluation of thyroid autoantibody Montelukast Sodium development with multiple regression analysis, the reference categories were CT60 CTLA-4 genotype, age, number of pregnancies, family history of AITD and cigarette smoking. For thyroid peroxidase antibodies, we established a significant contribution of CT60 CTLA-4 genotype (P < 0·04), while the effect of other factors was not confirmed. In thyroglobulin antibodies, no significant contribution of genetic or non-genetic factors was found. In PPT patients, 41 (54·7%) were hyperthyroid at presentation, while hypothyroidism was established in 34 (45·3%) patients. As presented in Table 3, the median value of thyroid peroxidase antibodies was significantly higher in the hypothyroid form of disease (P < 0·0001). Similarly, the median value of thyroglobulin antibodies was higher, although the difference was statistically insignificant.