4 (lane 3) showed absence of DNA band and only a smear of degraded DNA was observed. All the extracts except methanol showed observable protection of DNA Modulators intactness. Free radicals are known for DNA strand breaking and damage which eventually contributes to carcinogenesis, mutagenesis and cytotoxicity.16 Various researchers have reported the similar results and used plant extracts and fractions for DNA protection against oxidative damage.16 and 28 One of the interesting finding of present study was that ME did not show significant DNA protection activity which can be attributed to its inability to scavenge OH radicals (Fig. 2). It can be postulated from the results depicted in Fig. 5

that AAPH degraded BSA protein (lane 3). However, pre-treatment Y-27632 of H. isora fruit extracts effectively protected the protein from AAPH-induced

oxidation, which can be seen in terms of restoration of band intensity in the gel. These results hold significance and may have a positive role in inhibiting several stress or toxicity induced-protein oxidation. 26 All authors have none to declare. Authors thank the Principals of Modern College and Prof. Ramkrishna More College, Pune for encouragement and support to carry out this work. “
“Pyrroles and their derivatives exhibit different important biological activities, like antibacterial, antioxidant, cytotoxic and insecticidal R428 supplier properties.1, 2 and 3 Several five membered heteroaromatic systems like 1,2,4-triazole, 4-oxadiazole and 4-oxazolidinones having three hetero atoms at symmetrical nearly positions have been studied because of their interesting physiological properties.4, 5 and 6 They exhibit board spectrum

of pharmacological activities such as antiinflamatory,7 and 8 antiviral9 and antibacterial10, 11, 12, 13 and 14 activities. In view of the above mentioned pharmacological activities of pyrrole, 1,2,4-triazole, 4-oxidiazole and 4-oxaazolidinones, a number of the 2-substituted 3,5-dimethyl-2,4-diethoxy carbonyl pyrrole derivative have been synthesized containing above moieties. The reaction sequence leading to the formation of desired heterocyclic compounds are outlined in Scheme 1. The starting material 3,5-dimethyl-2,4-diethoxy carbonyl pyrrole (1) was prepared,15 refluxed with hydrazine hydrate to give 2- (3′, 5′ dimethyl-4′-ethoxy carbonyl pyrrole) acid hydrazide (2) was then refluxed with different iso-cyanate16 and 17 in presence of ethanol for 8 h. The isosemi-carbazide (3a–g) was heated with alkaline ethanolic solution for 3 h afforded 5-(3′,5′-dimethyl-4′-ethoxy carbonyl pyrrole)-4-phenyl-3-hydroxy-1, 2, 4-triazole (4a–g). 5-(3′,5′-dimethyl-4′-ethoxy carbonyl pyrrole)-1-phenyl amino-1,3,4-oxadiazole (5a–g) were obtained by cyclization of (3) by stirring it with conc. H2SO4, for 4 h.