The authors report no financial or other conflict of interest relevant to the subject of this article. Table S1. Strains and plasmids. Table S2. Oligos. “
“An Amazon soil microbial community metagenomic fosmid library was functionally screened for β-glucosidase activity. Contig analysis of positive clones revealed the presence of two ORFs encoding novel β-glucosidases, AmBGL17 and AmBGL18, from the GH3 and GH1 families, respectively. Both AmBGL17 and AmBGL18 were functionally identified

as β-glucosidases. The enzymatic activity of AmBGL17 was further characterized. AmBGL17 was tested with different substrates and showed highest activity on pNPβG substrate with an optimum temperature of 45 °C and an optimum pH of 6. AmBGL17 showed a Vmax of 116 mM s−1 and Km of 0.30 ± 0.017 mM. This is the first report of β-glucosidases from an Amazon soil microbial community using a metagenomic approach. “
“Forty-five selleck chemical bacterial strains that produced diffusive pigments were isolated from 40 soil samples. Maximum pigment production was from a Streptomyces kathirae strain designated SC-1. The diffused pigment was characterized by UV–visual and infrared spectroscopy, MS and 1H nuclear PLX4032 magnetic resonance imaging, and was confirmed as melanin. This may be the first report of melanin production by S. kathirae. To enhance melanin production, the culture medium was optimized by

conducting a series of batch fermentations in a defined medium, and the results were analysed statistically using a response surface method. The optimal culture medium comprised 3.3 g L−1 amylodextrine, 37 g L−1 yeast extract, 5 g L−1 NaCl, 0.1 g L−1 CaCl2 and 54.4 μM CuSO4. The pH of this medium was 6.0. Under optimal conditions, the melanin concentration was maximized at 13.7 g L−1, c. 8.6-fold

higher than obtained in suboptimal medium. To our knowledge, the results provide novel data on melanin fermentation, and identify an excellent candidate for industrial-scale microbial fermentation of melanin. “
“Clostridium difficile is the primary Phosphatidylinositol diacylglycerol-lyase cause of nosocomial diarrhoea in healthcare centres of the developed world. Only a few antibiotics are available for treatment, and relapses are common in patients undergoing antibiotic therapy. New approaches are required to reduce reliance on antibiotics, the use of which represents a primary risk factor for development of C. difficile infections. Supplementation of the gut flora with probiotics represents a key area for producing more successful treatment options for C. difficile infection (CDI). In this study, spores of B. subtilis have been evaluated as a potential probiotic treatment against CDI. Using a murine model of infection, we demonstrate that oral administration of B. subtilis spores can attenuate the symptoms of infection.