However, in relation to solid food, which provided the nutritional intake of Ca and P, nutritional pairing was achieved. Even though controlling

the amount of alcohol consumed by the animals was achieved, another limitation of our experiment was the absence of evaluating blood alcohol concentrations. Other studies could include the measurement of this in their experimental designs. Finally, it is important to consider that our work was limited to Ca/P ratio analysis. Without other parameters of evaluation, it was only possible to correlate the results with other searches. Broader studies are therefore required to better verify the potential relevance of these results in dental practice. It can be concluded that ovariectomy associated with alcohol consumption of 20% led to a significant decrease in Ca/P ratios within the region of alveolar bone crest in rats. Pictilisib E7080 nmr The authors

acknowledge support from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brazilian Federal Agency for Support and Evaluation of Postgraduate Education), native English speaker V. Hegenberg and statistician consultant, J. Adans. Funding: Adriana M.P.S. Marchini received a scholarship from the Brazilian governmental research agency, CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior). Competing interests: The authors report no conflict of interest relating to this study. Ethical approval: This study was approved by the ethics committee of São José dos Campos School of Dentistry, Meloxicam State University

of São Paulo – UNESP (Protocol No. 021/2008-PA/CEP). “
“Forensic identification of victims is essential for humanitarian reasons, but also for civil or criminal investigations. Identification of a corpse is essentially based on anthropology, odontology, fingerprints, radiology, and/or DNA typing.1 However, it can be complicated when the corpse is old, completely destroyed from mass disaster or putrefactive, skeletonized, drowned, or burned. In these cases, identification is usually difficult1, 2 and 3 since the elements used by pathologists, anthropologists and/or odontologists (such as fingerprints, sexual characteristics, physical constitution, ethnic group, stature and/or dental arch) can be modified by degradation, hampering a conclusive result. Given this scenario, forensic specialists looking for better preserved tissues to obtain DNA with good quality and amount4 and 5 have turned to DNA analysis.6, 7, 8 and 9 An excellent alternative is the use of cells from inside molar and pre-molar teeth. Regarding the molar and pre-molar mineralized inert structure, size, and location,4, 10, 11, 12, 13 and 14 they preserve cells with high molecular weight DNA for longer periods even when the body is in an advanced state of decomposition.