In general, recurrent disease behaves more as a developing cancer rather than the che mical stress responses required by primary disease. Conclusion CSCs targeting is a potential avenue through which treat ment of recurrent, chemoresistant ovarian cancer may be improved. This is complicated by the similarities between cancer and non cancer stem cells and our poor under standing of recurrent ovarian disease. We have identified the early events of stem cell differentiation as a key area of difference between cancer and non cancer stem cells. Furthermore, we have highlighted the association of a p53 p21 related cancer stemness signature within ovarian disease. Our data suggests that a stem cell involved in development of recurrent disease employs different mechanisms of tumorigenesis.

Our study suggests that it may be possible to target early differentiation events in CSCs without damaging non cancer stem cells, which would have broad implications for treatments. Our data indicates that such therapies should be independently tai lored for primary and recurrent ovarian disease. CSC tar geting during treatment of primary disease selleck chemical FR 180204 is likely to have a negative impact on recurrent tumorigenesis. CSC targeting in recurrent disease should be developed with consideration to independent mechanisms. Development of strategies to achieve this will continue in our group. Background The ovarian surface epithelium is a single layer of squamous to cuboidal cells surrounding the ovary that exhibits both epithelial and mesenchymal characteristics.

During monthly ovulations, the primary function of the OSE is to remodel the ovarian surface and selleckchem under lying extracellular matrix to allow for rupture of a ma ture follicle. Following oocyte extrusion, the OSE proliferates to heal the wound in the surface of the ovary. OSE have receptors for steroid hormones and growth factors, both of which are found in abundance in follicular fluid released during ovulation. In particu lar, the OSE has been shown to express insulin receptor and insulin like growth factor receptors, additionally, at high concentrations insulin can signal through IGF1R or through hybrid receptors of IR and IGF1R. Activation of IR or IGF1R by ligand binding activates downstream signaling pathways including the phosphatidylinositiol 3 kinase and mitogen activated protein kinase pathways.

In turn, pro liferative and anti apoptotic pathways are activated, including Akt, glycogen synthase kinase 3 B, Bcl2, and Bad. In immortalized OSE cell lines and many primary cell cultures, insulin is a critical component of the culture medium required for propagation of the cells, however, the ovary is not a classically insulin responsive tissue. Crosstalk can occur between IR and IGF1R signal ing when high concentrations of insulin initiate signaling through IGF1R.