proportion and var iety of expressed genes involved Vandetanib IC50 in the defense responses and the responses to jasmonic acid ethyl ene dependent systemic resistance. In both cases class I chitinases appeared to be responsible for much of the observed dif ferential expression. Lipoxygenases appeared to be re sponsible for differential expression in the category response to JA stimulus, which is consistent with the result in the category fatty acid biosynthesis. On the other hand, GO analysis indicated no significant differ ences between the compared treatments Inhibitors,Modulators,Libraries in transcript abundances involved in transport, carbohydrate metab olism, signal transduction, translation, transcription, ET and SA pathways. The distribution of Unitrans 2 ESTs between the differ ent treatments annotated against the plant taxonomic UniProt database is shown in the Venn diagrams of Figure 3.

Focusing on the analysis of the egg induced treatment and the mixed library EF F, the pairwise intersections Inhibitors,Modulators,Libraries between the C, E and EF treatments are about 30% of the Unitrans. When including data from the other treatments, half of the Unitrans for the EF or F treatments overlap with MeJA. Interestingly around 90% of the C Inhibitors,Modulators,Libraries and F treatment Uni trans overlap with the those from the mixed Inhibitors,Modulators,Libraries sample EF F. This suggests that many of the assignments that are apparently unique to one treatment may well be shared with other treat ments, but insufficient sequence coverage prevented de tection in these other samples. We have highlighted those transcripts assigned to the gene ontology category defense response in the Venn dia grams.

As expected, only a small num ber of Unitrans from the untreated plants were found to be assigned to this category. All Unitrans related to defense were detected in treatments that in clude induction by eggs. Here the Unitrans number increased Batimastat with the library size. Table 2 shows a list of Unitrans with predicted gene functions belonging to the GO category defense response. For visualization of metabolic pathways represented by gene transcripts, maps were reconstructed with the iPath software, using enzymes corresponding to the anno tated Unitrans. The enzymes are designated by the usual en zyme commission nomenclature. Cross comparisons among treatments demonstrate that most enzymes are only expressed in one of the two com pared treatments below.

Because library MG132 size had a strong influence on the extent of the annotated and mapped enzymes, we mapped the largest library, EF F, in which most transcripts of the other libraries occur. We used the 451 EC numbers of the EF F library to generate a meta bolic map to examine putative biochemical pathways present in feeding and egg induced U. minor, and also highlighted those putative enzymes preferentially expressed in egg induced plants. Enzymes associated with primary metabolism are predominant, whereas enzymes associated with secondary metabolism are much less prevalent. To elucidate the molecular basis for the biosynthesis of volatiles inv