The use of zebrafish in toxicity research can ultimately
learn more lead to the refinement or reduction of animal use. (C) 2012 Elsevier Inc. All rights reserved.”
“Background: Arterial bypass graft implantation remains the primary therapy for patients with advanced cardiovascular disease; however, there is no available synthetic small-diameter vascular graft.
Methods: Tissue-engineered vessels were grown from human smooth muscle cells that were seeded on a biodegradable scaffold using a biomimetic perfusion system. The human tissue-engineered vessels (hTEV) were decellularized by a two-step process using a combination of detergents and hypertonic solutions. The mechanical characteristics were assessed by suture retention strength and burst pressure. The decellularized hTEV were implanted as aortic interpositional grafts in nude rats to evaluate in vivo performance as an arterial graft over a 6-week period.
Results: The human tissue-engineered structure formed a vessel composed of smooth muscle cells and the extracellular
matrix proteins, including collagen. After decellularization, the collagen matrix remained intact while the cellular components were removed. The mechanical strength of the hTEV after decellularization click here was similar to human vein in vitro, with a burst pressure of 1,567 +/- 384mmHg (n = 3) versus 1,680 +/- 307mmHg for human saphenous vein. The hTEVs had a high patency rate (four of five grafts) without evidence of rupture or aneurysm over a 6-week period as an aortic interpositional graft in
a nude rat model. Histologic analysis showed a thin neointima with a confluent endothelium and a subendothelial layer of smooth muscle cells on the explanted tissue-engineered vessels. Transmission electron microscopy on the explanted tissue demonstrated elastin formation in the neointima and intact residual collagen fibers from the tissue-engineered vessel.
Conclusions: The hTEV had a high patency rate and remained mechanically stable as an aortic interpositional graft in a Palbociclib purchase nude rat. The vessel supported the growth of a neointima with endothelial cells and smooth muscle cells. The host remodeling suggested the engineered matrix had a positive effect to create a regenerated vascular graft. (J Vasc Surg 2012; 55: 790-8.)
Clinical Relevance: The demand for alternative arterial conduits is due to the poor clinical efficacy of existing synthetic grafts for small-diameter artery applications, with many patients lacking adequate saphenous vein. We showed that a vessel culture system could produce a human vascular graft that could function as an arterial conduit in a small-diameter animal model. The decellularization process for the human tissue-engineered vessels expands the clinical potential by generating an allogeneic graft that is readily available for implantation.