These data are summarised in Supporting information Figs S1 and S

These data are summarised in Supporting information Figs S1 and S2. Consistent with the data for the whole striatum, above, the performance in the corridor, apomorphine and amphetamine tests showed strong correlation SAHA HDAC mouse with the extent of denervation

in both dorsal and ventral striatum (corridor: R2 = 0.30, P < 0.001 and R2 = 0.57, P < 0.0001; apomorphine rotation: R2 = 0.30, P < 0.001 and R2 = 0.56, P < 0.0001; amphetamine rotation: R2 = 0.48, P < 0.0001 and R2 = 0.33, P < 0.0001, respectively), while the impairments in the stepping and cylinder tests were poorly correlated with any of these parameters (R2 = 0.15, P < 0.05, or less). The correlations with TH+ cell loss in SN or VTA, analysed separately, showed a similar pattern as for TH+ innervation density (right-hand panels in supporting Figs S1 and S2). The results summarised in Fig. 5 suggest that the impairments seen in the different tests are poorly correlated. To corroborate this impression further we studied how the scores in the five different tests correlated with each other. The behavioural impairments observed in the corridor task were well correlated with the apomorphine rotation scores (R2 = 0.73, P < 0.0001; Fig. 6D), and to a lesser extent also with the buy MLN0128 impairments observed

in the stepping test (R2 = 0.43, P < 0.0001; Fig. 6B), but not with the scores recorded in the cylinder and amphetamine rotation tests (R2 = 0.09, P = 0.09, n.s; R2 = 0.10, P < 0.05, respectively; Fig. 6A and C). It is notable that the amphetamine and apomorphine rotation scores showed no correlation to one another (R2 = 0.09, P = 0.06, n.s; Fig. 6G), and that the impairments seen in the cylinder test were modest overall, and were poorly correlated with the performance in any of the other tests used (R2 ≤ 0.16). One of the main

purposes of the present study was to develop criteria for the in vivo selection of well-lesioned 6-OHDA-lesioned mice based on their performance in selected behavioural tests. In order for a test to be of useful for this purpose it must be able to differentiate between animals with various degrees of lesion. To pursue this further the 40 6-OHDA-lesioned mice included in the present study very were allocated to three subgroups, based on the extent of striatal denervation recorded in each animal: severe lesion (80–100% denervation; example shown in Fig. 3A), intermediate lesion (60–79% denervation; Fig. 3B) and mild lesion (< 60% denervation; Fig. 3C). (For an overview of TH+ cell loss, striatal denervation and behavioural deficits of the mice in the three subgroups, see Table 1.) We then compared the behavioural deficits of these three subgroups to see whether each of the tests could discriminate between the extent of lesion (Fig. 7).

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