This concept is supported by the finding that 15% of melanized neurons in the human SNpc no longer

express tyrosine hydroxylase (TH; the rate-limiting enzyme in DA synthesis), but remain morphologically intact:4 This concept of the suffering, ie, metabolically compromised, neuron is important in pathophysiological and therapeutic terms, since it suggests that, a subpopulation of nigral DA neurons are amenable Inhibitors,research,lifescience,medical to restorative therapies. Figure 1. Axial cut of human mesencephalon. The substantia nigra pars compacta (SNpc) can be identified by tyrosine hydroxylase (TH) immunostaining reflecting the presence of dopaminergic (DA) neurons. Cell loss in the parkinsonian SNpc can be appreciated macroscopically … After a general outline on the potential and limitations of human postmortem studies in PD, 1 will explore major questions regarding etiology, pathogenesis, and treatment of PD with reference to human postmortem studies. The role of human postmortem Inhibitors,research,lifescience,medical studies in PD research There has been considerable debate over the importance Inhibitors,research,lifescience,medical of human postmortem studies in PD research. This controversy is based on the many limitations of postmortem research. Human postmortem studies in PD suffer from tissue confounds of aging, end-stage disease, and chronic treatments. Moreover, human postmortem

studies cannot answer the question of whether the changes observed are a cause or a consequence Inhibitors,research,lifescience,medical of neuronal death in PD. On the other hand, no animal model to date perfectly replicates PD etiopathogenesis, and the molecular weight calculator anatomical organization of the nigrostriatal system differs considerably between humans and lower species. Thus, human postmortem material remains the gold standard for (i) formulating hypotheses for subsequent, testing in in vitro and in vivo PD models (cell culture, Inhibitors,research,lifescience,medical yeast, Drosophila, rodent, and primates models of the disease, based on toxins and/or genetic manipulations); and (ii) verifying hypotheses derived from

experimental PD models with regard to their validity in the human disease. This review will emphasize the interaction of findings from postmortem and experimental PD studies. Genes and PD The etiology of sporadic PD remains unknown. It is generally believed that, sporadic PD is the result, of Sirolimus complex interactions between genetic susceptibility and environmental factors. Entinostat In both cases, postmortem studies serve to confirm rather than to generate new hypotheses, with a few notable exceptions. Genetics is a rapidly growing field in PD research. Three major mutations have been identified to date in affected kindreds: oc-synuclein or Park15; parkin or Park26; and DJ-1 or Park77 A fourth mutated gene product, UCHL1 (Park5), is associated with gene expression; it may not be able to provoke a parkinsonian syndrome alone,8 but may be a susceptibility gene.