5). Data confirm that infection significantly affected weight compared with non-infected animals throughout the 8 day
period (p < 0.05). The data also show a single treatment of infected ferrets with 244 DI virus resulted in a greater overall weight gain that was seen with the infected control animals (p < 0.05). This indicates that while the treated animals experienced a transient weight loss on day 3 ( Fig. NLG919 in vivo 1a), this was less than was seen with the infected control group, and that treated animals subsequently gained weight at a greater rate than did the control infected animals. In contrast the repeated measures ANOVA showed that while multiple (10) treatments with oseltamivir resulted in a reduced weight loss when compared with the infected control group ( Fig 1a), this was not significant at the 5% level. ZD1839 concentration The repeated measures ANOVA identified day 3 post-infection as the time at which the greatest difference between
either of the two treatments and the control infected group occurred, with DI virus providing the more effective amelioration of weight loss. Separate analysis of data using a one tailed unpaired t test was in agreement with the repeated measures ANOVA. The t-test showed that a single treatment with 244 DI virus at 2 h prior to infection significantly protected ferrets from A/Cal-associated weight loss on days 3 and 4 ( Fig. 1b). By t-test oseltamivir, given at 2 h prior to infection and overall twice daily for 5 days, did not significantly reduce weight loss compared to the untreated infected group on days 3 and 4 ( Fig. 1b). Fig. Racecadotril 2a shows the mean daily temperatures for the groups of ferrets following infection. Control A/Cal-infected ferrets developed a pronounced fever spike at 3 days after infection (circled). Fever was reduced by both 244 DI virus and oseltamivir treatments. Though the reduction in temperature on day 3 post infection with either treatment was clearly evident, neither was statistically significant at the 95% level (p = 0.09 and p = 0.07 for treatment with 244 DI virus or oseltamivir treatment, respectively). This was due to one ferret in the control A/Cal-infected group that recorded
a non-elevated temperature, as omission of this data point gave p values of 0.02 and 0.04 for treatment with 244 DI virus or oseltamivir, respectively ( Fig. 2b). There was no statistical difference in the day 3 temperatures in infected ferrets treated with 244 DI virus or oseltamivir (p = 0.32). Ferrets were monitored for sneezing and nasal discharge, both typical respiratory signs of influenza. Analysis of data collected twice daily (morning and afternoon) over the 14-day duration of the study showed that treatment with 244 DI virus significantly (p = 0.009) reduced the score compared with the infected A/Cal control group by 1.7-fold ( Fig. 3). Oseltamivir treatment gave no significant reduction in respiratory disease ( Fig. 3).