In our study, the expressional level of Annexin A1, A2, A3, A5 and A7 increased E7080 ic50 compared with the normal liver tissue. Annexins consist of a conserved protein family. Annexin A2 is closely associated with cell division regulation and tumor growth, and is deregulated in many tumors[56, 57]. Two Annexin A2 molecules bind to the long chains of p11/S100A10 dimers through its N-terminals, form the isotetramer, regulating the reactions of Annexin A2 and membranes and actin in cortical areas, and the distribution of recirculating endosomes[58]. In addition, S100A10 and Annexin A2 form isodimers, prompting the invasion and metastasis

of the tumor by activating plasminogen[59]. In the present study, the expression level of S100a10, S100a11, S100a6, CP673451 order S100a8 and S100a9 increased from cirrhosis to metastatic process when compared with the normal liver. S100A8/A9 form the compounds that play a role in inducing apoptosis in tumor cells. S100A8/A9 at low concentrations prompts growth activity,

the phosphorylation of MAPK pathway and NF-κB is activated in cells after S100A8/A9 treatment. The majority of HCCs slowly unfold against a background of chronic hepatitis and cirrhosis, which can be considered AZD5582 as preneoplastic conditions of the liver. Chronic hepatitis is characterized by persistent inflammation, cytokine and oxidative stress-mediated hepatocyte death and active proliferation of residual hepatocytes to replace the lost parenchyma[1, 60]. During the process of hepatocarcinogenesis in rat models, chronic inflammation precedes cirrhosis. Epidemiology studies showed that chronic inflammation increased the risk of tumors, and the microenvironment of tumorigenesis resembles the reaction of inflammation to injury in many

ways[61]. In the tumor microenvironment, the chemotactic factors and receptors mediated angiogenesis, recruited cells, prompting cellular survival and proliferation. On the other hand, oxidative stress occurred in inflammatory processes. The inflammatory cells and tumor cells both produce free radicals and soluble factors such as arachidonic acid, cytokines and chemotactic factors, seubsequently producing reactive oxygen. All these factors strongly recruit the inflammatory cells to produce LY294002 cytokines, which promotes a vicious cycle. The intermediate products of active oxygen oxidize DNA directly or interfere with DNA repair. These oxides activate protein, carbohydrate and lipids quickly, the derived products interfere with inter- and intracellular homeostasis, favoring DNA mutation. Thus, the chronic inflammation prompts the malignant transformation of cells[62]. Chronic inflammation also favors angiogenesis[63]. In the present study, many DEGs are related to inflammation reaction, immune reaction and stress.