Initially we developed two sets of outbreak reconstructions, one constructed INCB018424 chemical structure using the regional lodgepole pine chronology as the non-host and a second constructed using the regional ponderosa pine chronology as the non-host. The average correlation coefficient between the reconstructions was 0.60 (ranging from 0.42 to 0.83) (Fig. 2a), indicating good correspondence between non-hosts and providing confidence that either could be used in the outbreak analyses. The outbreak reconstructions for each non-host are plotted for Site 5 (Fig. 2b), which had an average correlation coefficient between reconstructions (r = 0.58), illustrating that overall the two non-hosts produced similar outbreak histories in terms of timing
and duration. The Alectinib supplier most significant difference between the two reconstructions was the outbreak intensity. Reconstructions based on lodgepole pine generally had a higher ratio of trees meeting the outbreak
parameters than those based on ponderosa pine (e.g., Fig. 2b). This pattern was consistent for all sites suggesting that WSB outbreak reconstructions based on ponderosa pine are likely to be more conservative. Nonetheless, we chose to use only the regional ponderosa pine chronology to construct our final WSB reconstruction as it extended further back in time (>400 years). Our WSB reconstructions show that outbreaks have occurred in the Cariboo Forest Region for the past 300–400 years (Fig. 3). These outbreaks have varied in intensity and duration at individual sites, but at times were highly synchronous across the study area (Fig. 3 and Fig. 5). The sites with the longest outbreak reconstructions (FR, RS, FC, S6, ML and CM; Table 1) all 4-Aminobutyrate aminotransferase recorded outbreaks in the early-1600s and 1630s, where 80–100% of trees recorded outbreaks (Fig. 3). From the 1650s to the early-1700s site-specific outbreaks occurred at generally low levels. In the 1720s a synchronous, moderate outbreak occurred at all sites (Fig. 3). In the 1770s a high intensity (60–80% of trees) outbreak occurred at nearly all the sites, with the exception of FC and S1 (Fig. 3). The 1800s were characterized by a
period of predominately stand-specific outbreaks of variable intensity until the late-1800s when all the sites recoded a severe outbreak (80–100% of trees) (Fig. 3). Our reconstructed outbreak history was compared with survey records from the 20th and 21st centuries. Ninety-one percent of the Douglas-fir stands examined in this study record outbreaks from the late-1930s to mid-1940s (Fig. 3). Although few records exist in the study area prior to 1994, documented outbreaks in the mid-1930s to mid-1940s to the south and west closely coincide to our reconstructed outbreaks (Harris et al., 1985). The 1974 WSB outbreak observed by Erikson (1992) near the FC site chronology (Fig. 1) appears at 64% of our sites, albeit as a low intensity event that impacted from 20% to 40% of the trees at sites recording the outbreak (Fig. 3).