These potentially beneficial effects of green tea selleck inhibitor are attributed to catechin compounds, particularly EGCG, which is the most abundant and extensively studied catechin compound of green tea [12, 13]. The overall medicinal effects of green tea observed thus far, are focused on combined activities of several compounds in green tea rather than that of a single compound. In addition, most studies have PF-562271 research buy investigated the different synergistic bioactivities of all compounds present in tea extracts or have been focused mainly on the role of EGCG. Therefore, the present study was designed to elucidate the role of the anticancer activity of single compound i.e. CH (Figure 1) at the molecular level.
Figure 1 Molecular structure of catechin hydrate. Materials and methods Catechin Hydrate-A compound of Catechins Catechin is a polyphenolic flavonoid which has been isolated from a variety of natural sources including tea leaves, grape seeds, and the wood and bark of trees such as acacia and mahogany. Catechin is
a more potent antioxidant than ascorbate or this website α-tocopherol in certain in vitro assays of lipid peroxidation. Catechin inhibits the free radical-induced oxidation of isolated LDL by AAPH [14]. Catechins and other related procyanidin compounds have antitumor activity when tested in a two-stage mouse epidermal carcinoma model employing topical application. Following is the structure of (+)-Catechin hydrate. Preparations of CH 100 mg CH was dissolved in 10 mL DMEM medium (10% FCS) to obtain stock solution and was further diluted in medium to obtain desired concentrations. Maintenance of MCF-7 Cells The MCF-7 breast cancer cell line was a kind gift from Dr. M. A. Akbarshah at the Mahatma Gandhi-Doerenkamp Center (MGDC) for Alternatives to Use of
Animals in Life Science Education, Bharathidasan University, India. The cell line was maintained and propagated in 90% Dulbecco’s Modified Eagle’s Medium (DMEM) containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. Cells were cultured as adherent monolayers (i.e., cultured at ~70% to 80% confluence) Galeterone and maintained at 37°C in a humidified atmosphere of 5% CO2. Cells were harvested after being subjected to brief trypsinization. All chemicals used were of research grade. Viability of Cells Cell viability was assayed using a trypan blue exclusion test as explained earlier with slight modifications[15]. Toxicity and Cell Proliferation Assays The Cell Titer Blue® viability assay (Promega Madison, WI) was performed to assess the toxicity of different concentrations of CH on MCF-7 cells. The assay was performed according to the manufacturer’s instructions. Briefly, MCF-7 cells (2 × 104 cells/well) were plated in 96-well plates and treated with 0 μg/mL CH and 160 μg/mL CH for 24 hours. Then, 40 μL of the Cell Titer Blue solution was directly added to the wells and incubated at 37°C for 6 hours.