G�CH) Proteins involved in triglyceride metabolism such as DGAT2 and PLIN displayed zone 3 to zone 1 distributions. I�CP) Several proteins facilitating phospholipid metabolism, specifically CHKA, PCYT1B, PEMT, PCYT2, GPAT2, PLA2G15, PLA2G4F selleckbio and PLA2B1 all displayed strong zonation. In each instance, except for PCYT2, the strongest staining was in zone 3. (DOCX) Click here for additional data file.(16M, docx) Figure S4 Portal tracts imaged by MALDI-IMS display robust levels of PC 320. Photomicrographs of H&E stained sections from each specimen (A, C, E) and corresponding MALDI IMS images of selected liver specimen obtained from subjects with normal (A�CD) and NASH (E�CF) histologies. (B, D, F) MALDI image of m/z 772.52 PC 320 [M+K]+. Ion intensity color scale for all ion images is shown at the top of the figure.
Scale bar=500 ��m. (DOCX) Click here for additional data file.(2.3M, docx) Table S1 Fragmentation and LC retention time information. (DOCX) Click here for additional data file.(14K, docx) Table S2 Lipids Identified and Quantified in Hepatic Extracts by LC ESI-MS/MS. (DOCX) Click here for additional data file.(18K, docx) Table S3 List of proteins identified in normal human liver and their expression profile in the Human Protein Atlas. (DOCX) Click here for additional data file.(25K, docx) Methods S1 (DOCX) Click here for additional data file.(23K, docx) Acknowledgments The authors thank Dr. Yu Shyr, Vanderbilt Center for Quantitative Sciences, for additional statistical support. We are indebted to Dr.
Kay Washington, Vanderbilt Department of Pathology, for assistance in the pathological assessment of the liver specimens. We are grateful for the guidance and expertise of Dr. David Hachey, Dr. Wade Calcutt and the Vanderbilt Mass Spectrometry Research Center in assisting with nano LC-MS analyses. We thank Dr. Lynne Lapierre, Vanderbilt Epithelial Biology Center, for assistance with immunohistochemistry. We are thankful to Jamie AV-951 Allen, Vanderbilt Mass Spectrometry Research Center, for assistance with sample preparation. We thank Clark M. Murray for assistance in the fragmentation analyses of human tissues and Caleb Huber for assistance in MALDI IMS dataset curation. Fun
We describe a case of moderate hand�Cfoot syndrome and nail toxicity simultaneously occurred in a 69-year-old man with advanced gastric cancer (cT4-N3-M1, G3) previously treated with numerous antineoplastic agents, including PELFw (5-fluorouracil, epidoxorubicin, leucovorin, and cisplatin) and taxoids. Due to a recalcitrant course, the above therapeutic agents were discontinued for 4 weeks and he was given solely capecitabine 1250 mg twice daily for 2 weeks at 3-weekly intervals.