A standard z-score was used to identify hits from the RNAi screen. The z-score was based on a raw score defined as z = (x-μ)/σ, where x is a reporter gene activity from a single well,
μ is the mean reporter gene activity calculated for entire plate including non-silencing shRNA MK-8776 concentration samples, and σ is the standard deviation of the entire plate. Acknowledgements We thank Hongzhao Tian for technical assistance. This work was supported by a LANL Laboratory-Directed Research and Development Exploratory Research Grant and by the National Center for Research Resources and the National Institute of General Medical Sciences of the National Institutes of Health through Grant Number P41-RR01315, “The National Flow Cytometry Resource”. The funding agencies had no role in the design of the experiments, analysis of the data, or writing of the manuscript. References 1. Cornelis G: Yersinia type III secretion: send in
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